Correlation between elements accumulation and active components in velamen and stem bark of Periploca sepium / 中草药

Objective: To study the dynamic variation of Mn, Cu, Zn, Fe, K, Ca, and Mg in velamen and stem bark of Periploca sepium and their correlation with the elements such as periplocin and 4-methoxysalicylic aldehyde in different harvesting periods, and to reveal dominant factors on the accumulation of two active components. Methods: The contents of periplocin and 4-methoxysalicylic aldehyde were detected by FAAS and electric heating wet digestion, and the data were analyzed by SPSS 19.0. Results: The seven elements were varied widely in velamen and stem bark. The variation trends of Ca and periplocin, Fe and 4-methoxysalicylic aldehyde in velamen were similar, equally, those of Fe and 4-methoxysalicylic aldehyde, Ca and Mg in stem bark were similar and like double peaks. Periplocin has highly significant positive correlation with Ca in velamen and significant negative correlation with Zn in stem bark. To 4-methoxysalicylic aldehyde, when in velamen, it has highly significant positive correlation with 4-methoxysalicylic aldehyde and Fe in stem bark, significant negative correlation with Mn in stem bark, and significant positive correlation with Fe in velamen; when in stem bark, it has highly significant positive correlation with Fe in both velamen and stem bark. Conclusion: Ca, Zn, Fe, and Mn are dominant elements of accumulation, the key factors for periplocin are Ca in velamen and Zn in stem bark, and Mg in velamen is indierect acting factors; The key factors for 4-methoxysalicylic aldehyde are Mn in stem bark and Fe, Ca and Mg in stem bark are indirect acting factors.

Lack of Mutagenicity Potential of Periploca sepium Bge. in Bacterial Reverse Mutation (Ames) Test, Chromosomal Aberration and Micronucleus Test in Mice

OBJECTIVES: The root barks of Periploca sepium Bge. (P. sepium) has been used in traditional Chinese medicine for healing wounds and treating rheumatoid arthritis. However, toxicity in high-doses was often diagnosed by the presence of many glycosides. The potential mutagenicity of P. sepium was investigated both in vitro and in vivo. METHODS: This was examined by the bacterial reverse mutation (Ames) test using Escherichia coli WP2uvrA and Salmonella typhimurium strains, such as TA98, TA100, TA1535, and TA1537. Chromosomal aberrations were investigated using Chinese hamster lung cells, and the micronucleus test using mice. RESULTS: P. sepium did not induce mutagenicity in the bacterial test or chromosomal aberrations in Chinese hamster lung cells, although metabolic activation and micronucleated polychromatic erythrocytes were seen in the mice bone marrow cells. CONCLUSIONS: Considering these results, it is suggested that P. sepium does not have mutagenic potential under the conditions examined in each study.